{"id":1041,"date":"2022-05-30T14:24:32","date_gmt":"2022-05-30T12:24:32","guid":{"rendered":"https:\/\/norfab.no\/technology\/steponeplus-real-time-pcr\/"},"modified":"2024-04-30T11:52:58","modified_gmt":"2024-04-30T09:52:58","slug":"steponeplus-real-time-pcr","status":"publish","type":"technology","link":"https:\/\/norfab.no\/technology\/steponeplus-real-time-pcr\/","title":{"rendered":"StepOnePlus real-time PCR"},"content":{"rendered":"

StepOnePlus is our machine for real-time\/quantitative PCR. <\/p>\n

The StepOnePlus can do both real-time runs or end-point runs.<\/p>\n

The StepOnePlus is connected to a laptop with the StepOne software. It is also possible to run it as a stand-alone system.<\/p>\n

 <\/p>\n

The system has four filters that can detect these fluorophores:<\/p>\n\n\n\n\n\n\n\n\n
Filter<\/th>\nDye<\/th>\nemission<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\n\n

FAM<\/p>\n

SYBR Green<\/p>\n<\/td>\n

~ 520 nm<\/td>\n<\/tr>\n
2<\/td>\n\n

JOE<\/p>\n

VIC<\/p>\n<\/td>\n

~ 550 nm<\/td>\n<\/tr>\n
3<\/td>\n\n

TAMRA<\/p>\n

NED<\/p>\n<\/td>\n

~ 580 nm<\/td>\n<\/tr>\n
4<\/td>\nROX<\/td>\n~ 610 nm<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

Extensive manuals for the following types of experiments are found in the biology lab's index file:<\/p>\n

    \n
  • Standard curve experiments<\/li>\n
  • Relative standard curve and comparative CT<\/sub> experiments<\/li>\n
  • Genotyping experiments<\/li>\n
  • Precence\/absence experiments<\/li>\n<\/ul>\n","protected":false},"featured_media":5942,"template":"","labs":[4],"tech_cat":[16],"acf":[],"_links":{"self":[{"href":"https:\/\/norfab.no\/wp-json\/wp\/v2\/technology\/1041"}],"collection":[{"href":"https:\/\/norfab.no\/wp-json\/wp\/v2\/technology"}],"about":[{"href":"https:\/\/norfab.no\/wp-json\/wp\/v2\/types\/technology"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/norfab.no\/wp-json\/wp\/v2\/media\/5942"}],"wp:attachment":[{"href":"https:\/\/norfab.no\/wp-json\/wp\/v2\/media?parent=1041"}],"wp:term":[{"taxonomy":"labs","embeddable":true,"href":"https:\/\/norfab.no\/wp-json\/wp\/v2\/labs?post=1041"},{"taxonomy":"tech_cat","embeddable":true,"href":"https:\/\/norfab.no\/wp-json\/wp\/v2\/tech_cat?post=1041"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}