StepOnePlus is our machine for real-time/quantitative PCR.
The StepOnePlus can do both real-time runs or end-point runs.
The StepOnePlus is connected to a laptop with the StepOne software. It is also possible to run it as a stand-alone system.
The system has four filters that can detect these fluorophores:
Filter | Dye | emission |
---|---|---|
1 |
FAM SYBR Green |
~ 520 nm |
2 |
JOE VIC |
~ 550 nm |
3 |
TAMRA NED |
~ 580 nm |
4 | ROX | ~ 610 nm |
Extensive manuals for the following types of experiments are found in the biology lab's index file: